Applied Microbial Biotechnology

Semester Taught - Spring

Catalog Description

Credits: 3

The course will focus on quantification of microbial growth and microbially mediated degradation and transformation processes emphasizing the application of these tools to develop mathematical models for industrial processes for pollution control, waste treatment, biofuel and bioproducts production and bioremediation.


Bioprocess Engineering

Course Objectives

  • develop concepts and mathematical tools required to understand and analyze microbial growth, and microbially mediated  degradation and transformation processes,
  • quantify and analyze bioreactor operation and performance through mathematical process modeling and simulation,
  • learn about some industrially important bioprocesses used for waste treatment, biofuel production and bioremediation,
  • construct mathematical process models to simulate operation of these bioprocesses. 


Pratap Pullammanappillil
Office - 203 Frazier Rogers Hall
Telephone (352) 392-1864 x203
E-mail address: pcpratap@ufl.edu

Material/Supply Fees


Class Materials Required

There is no required textbook.  Material will be covered from original research papers and from the following books:

  1. Rittmann, B. E. and McCarty, P. L.  2001.  Environmental Biotechnology: Principles and Applications,  McGraw Hill, New York.
  2. Biochemical Engineering, Bailey and Ollis, 1986, McGraw Hill
  3. Bu'lock, J. and Kristiansen, B., eds.  1997.  Basic Biotechnology, Academic Press, New York.

Lecture notes will be handed out.

Course Outline

  • Introduction
  • Stoichiometry and energetics
  • Phenomenological models
  • Mechanistic models
  • Structured models
  • Mixed culture interactions
  • Mixed product fermentation and mixed substrate utilization
  • Attached growth
  • Mathematical modeling of suspended growth and attached growth bioreactors
  • Case studies (Anaerobic digestion, Activated sludge process, Composting, Phototrophic processes, Microbial corrosion, Microbial Fuel Cells, Bioremediation)


Grades will be based upon field trip reports, homework problems, and class participation.

A 91-100
A- 86-90
B+ 81-85
B 76-80
B- 71-75
C+ 66-70
C 61-65
C- 56-60
D+ 51-55
D 46-50
D- 40-45
E <40
Grading MethodPercentage
Homework 50%
Exam 20%
Project 30%

Attendance and Expectations: Attendance is expected at all lectures. It is expected that students will have knowledge and access to a numerical analysis software package. Homework is due within one week of when assigned or as indicated.

Make-up Exam Policy:  No make-up exams will be given except for valid medical reasons or unless prior arrangements have been made.

Academic Honesty

All students admitted to the University of Florida have signed a statement of academic honesty committing themselves to be honest in all academic work and understanding that failure to comply with this commitment will result in disciplinary action. This statement is a reminder to uphold your obligation as a UF student and to be honest in all work submitted and exams taken in this course and all others.

Accommodation for Students with Disabilities

Students requesting classroom accommodation must first register with the Dean of Students Office. That office will provide the student with documentation that he/she must provide to the course instructor when requesting accommodation.

Use of Library, Personal References, PC Programs and Electronic Databases

These items are university property and should be utilized with other users in mind. Never remove, mark, modify nor deface resources that do not belong to you. If you're in the habit of underlining text, do it only on your personal copy. It is inconsiderate, costly to others, and dishonest to use common references otherwise.

Software Use

All faculty, staff and students of the University are required and expected to obey the laws and legal agreements governing software use. Failure to do so can lead to monetary damages and/or criminal penalties for the individual violator. Because such violations are also against University policies and rules, disciplinary action will be taken as appropriate. We, the members of the University of Florida community, pledge to hold ourselves and our peers to the highest standards of honesty and integrity.

UF Counseling and Career Services

Resources are available on-campus for students having personal problems or lacking clear career and academic goals which interfere with their academic performance. These resources include:

  1. University Counseling and Wellness Center, 3190 Radio Road, Gainesville, FL 32611
  2. Career Connections Center, Reitz Union, 392-1601, career development assistance and counseling.