University of Florida

ABE 5442
Bioprocess Engineering

Semester Taught - Fall

Catalog Description

Credits: 3

The course will cover engineering principles, processes and techniques for using biological agents such as cells, enzymes or antibodies for the production of chemicals, food, biofuels and pharmaceuticals, and waste treatment.  The course will include stoichiometry and kinetics of reactions that employ biological agents; design, analysis and operation of reactors (fermentors); and product recovery and purification (downstream processing).


Life Sciences, Biological, Chemical or Environmental Engineering coursework

Course Objectives

The objectives of the course are to develop concepts and mathematical tools required to understand and analyze,

  • processes involved in production of chemicals, food, biofuels and pharmaceuticals using biological agents.
  • design and operation of reactors using biological agents
  • unit operations and processes for product recovery
  • economics of bioprocesses


Pratap Pullammanappillil
Office - 203 Frazier Rogers Hall
Telephone (352) 392-1864 x203
E-mail address:

Material/Supply Fees


Class Materials Required

Textbook required:

Title: Bioprocess Engineering Principles
Author: Pauline M. Doran
Publisher: Academic Press, 2007 (11th reprint)
ISBN-13: 978-0-12-220856-0

Recommended Reading:

  1. Bioprocess Engineering – Basic Concepts, Shuler and Kargi, Second Edition 2002, Prentice Hall
  2. Principles of Fermentation Technology by Stanbury, Whitaker and Hall, Second Edition 2003, Butterworth Heinemann.
  3. Biochemical Engineering, Bailey and Ollis, 1986, McGraw Hill

Course Outline

  • Material and Energy Balances
  • Stoichiometry and energetics of microbial growth and product formation
  • Principles of Enzyme Catalysis
  • Cell Growth
  • Heterogeneous Reactions
  • Operating Considerations for bioreactors
  • Bioreactor Design and Analysis
  • Analysis of mixed cultures
  • Downstream processing
  • Bioprocess Economics


Grades will be based upon field trip reports, homework problems, and class participation.

A 91-100
A- 86-90
B+ 81-85
B 76-80
B- 71-75
C+ 66-70
C 61-65
C- 56-60
D+ 51-55
D 46-50
D- 40-45
E <40
Grading Method Percentage
Homework 50%
Exam 20%
Project 30%

Attendance and Expectations: Attendance at all lectures is expected from all students at all times. Homework is due within one week of when assigned.

Make-up Exam Policy:  No make-up exams will be given except for valid medical reasons or unless prior arrangements have been made.

Academic Honesty

As a student at the University of Florida, you have committed yourself to uphold the Honor Code, which includes the following pledge: “We, the members of the University of Florida community, pledge to hold ourselves and our peers to the highest standards of honesty and integrity.”  You are expected to exhibit behavior consistent with this commitment to the UF academic community, and on all work submitted for credit at the University of Florida, the following pledge is either required or implied: "On my honor, I have neither given nor received unauthorized aid in doing this assignment." 
It is assumed that you will complete all work independently in each course unless the instructor provides explicit permission for you to collaborate on course tasks (e.g. assignments, papers, quizzes, exams). Furthermore, as part of your obligation to uphold the Honor Code, you should report any condition that facilitates academic misconduct to appropriate personnel. It is your individual responsibility to know and comply with all university policies and procedures regarding academic integrity and the Student Honor Code.  Violations of the Honor Code at the University of Florida will not be tolerated. Violations will be reported to the Dean of Students Office for consideration of disciplinary action. For more information regarding the Student Honor Code, please see:  

UF Counseling Services

Resources are available on-campus for students having personal problems or lacking clear career and academic goals which interfere with their academic performance. These resources include:

  1. University Counseling Center, 301 Peabody Hall, 392-1575, personal and career counseling;
  2. Student Mental Health, Student Health Care Center, 392-1171, personal counseling;
  3. Center for Sexual Assault/Abuse Recovery and Education (CARE), Student Health Care Center, 392-1161, sexual assault counseling;
  4. Career Resource Center, Reitz Union, 392-1601, career development assistance and counseling.